Queer Establishing Moment

A Queer Establishing Moment is the moment when a character is revealed to be LGBTQ+.

Western Animation
The Loud House: In the episode "L is for Love", the Loud siblings try to woo their crushes; the audience is led to believe that Luna's crush, Sam, is a boy, only for said boy to walk past the locker Luna put a note in and for a girl to open the locker instead.

My Little Pony: Friendship Is Magic: In "The Big Mac Question", Lyra and Bon Bon are seen proposing to each other in the background, revealing that they're lesbians.

The Owl House:

Tuca & Bertie: Tuca is revealed to be bisexual in "Bird Mechanics" when she is shown to see both male and female characters are prospective dates on the Sex Bus.