Effeminate Voice

Anime & Manga

 * Naruto:
 * Haku is an androgynous teenage boy voiced by a woman doing a feminine voice. When Naruto sees him dressed as a civilian in a woman's kimono, he thinks that Haku's a girl.
 * Orochimaru has a Creepy High-Pitched Voice. He's always been androgynous to the point where, due to the fact he uses other's bodies to stay young, he often veers into Ambiguous Gender.
 * In the 2003 anime of Fullmetal Alchemist, unlike the manga, Envy is a very androgynous male and uses a feminine voice. He occasionally subverts this trope, usually when upset, and switches to a more masculine voice.

Western Animation

 * Ed, Edd n Eddy:
 * Jimmy is a quiet boy who likes baking, playing with dolls, and hanging out with his best friend (and crush) Sarah. He has the softest and most high-pitched voice out of the males.
 * Edd has several stereotypically feminine attributes and has a higher-pitched voice than his friends. This is probably why Eddy's brother mistook him for Eddy's girlfriend in The Movie.
 * Him from The Powerpuff Girls is a Creepy Crossdresser with a Creepy High-Pitched Voice.
 * Sponge Bob Squarepants: SpongeBob is an adult keet with an extremely high-pitched voice that got even higher post-movie due to Vocal Evolution.