Butch Lesbian

Western Animation

 * Steven Universe:
 * Ruby is a butch lesbian: she's an alien who has No Biological Sex but she uses female pronouns, is very tomboyish, and is in a romantic relationship with Sapphire, another non-binary femme-presenting alien.
 * Mystery Girl from "Last One Out of Beach City" fits the bill appearance-wise, complete with flannel, and she seems interested enough in Pearl to give her her number.
 * Pearl herself very well may fit the bill, being heavily based on the masculine woman-loving-woman Utena Tenjou and her character development involving moving away from the traditional femininity forced on Pearls and into a more emasculate appearance. Her on-screen love interests include Rose Quartz, a fellow gem, and Mystery Girl, the aforementioned human who is very likely a butch lesbian herself. The collection of phone numbers she's seen with in A Single Pale Rose appear to all be from women as well, further establishing Pearl as a lesbian specifically (insofar as any gems are women to begin with).
 * The Topazes are large, masculine-looking (but not vocally) Gem guards who care very deeply for each other.