Lipstick Lesbian

Anime & Manga

 * Miss Kobayashi's Dragon Maid: Riko Saikawa is a girly girl (though she has a noticeable tomboy streak) who's in love with Kanna.

Western Animation

 * There has been much controversy over the possibility of lesbian subtext in Adventure Time, specifically between Princess Bubblegum and Marceline. If this is to be believed, Princess Bubblegum, who wears her hair long, likes to wear long dresses, and, um, is entirely pink would fit this trope.
 * The Owl House has Amity, who develops a crush on the tomboyish bisexual Luz.
 * Steven Universe: As a side note, in general, Gems are actually genderless despite going with she/her pronouns.
 * Sapphire, the composed, sweet, gentle Gem, who is in a committed relationship with Ruby.
 * Likewise for Pearl, who's also of the discount type. She's graceful, acts motherly to Steven, wears a ballerina-inspired outfit, and she had a complicated one-sided love for Rose Quartz. Her prototypical designs were slightly less feminine (in particular the one used in the pilot) however her personality characterized her as far more of a jerk. As of the season 4 episode "Last One Out of Beach City", she's scored the Mystery Girl's phone number and was obviously attracted to her.
 * Pink Pearl, AKA "Volleyball", is one of the more girly Pearls we've seen, and, as our Pearl puts it, she's "still got it bad" for Pink Diamond.