Queer Character, Queer Actor

Web Animation

 * Hazbin Hotel: Gay porn star Angel Dust is voiced by an asexual Michael Kovach.
 * Helluva Boss:
 * Pansexual imp Blitzo is voiced by a gay actor Brandon Rogers.
 * Millie's sister Sallie May is a trans woman and is played by trans voice actress Morgana Ignis.

Western Animation

 * In The Ghost and Molly McGee, one of Molly's teachers, Mrs. Roop, is voiced by Jane Lynch, who's a lesbian in real life.
 * The Loud House:
 * Bisexual actor Alyson Stoner voices Luna's girlfriend Sam.
 * Transgender actor Maddie Taylor voices transgender pageant host Dana Dufresne. In fact, her character transitioned with her.
 * Q-Force:
 * Real-life gay actors Sean Hayes, Matt Rogers and Gabe Liedman voiced the gay characters Steve Mayweather, Twink and Benji.
 * Real-life lesbian actresses Wanda Sykes and Fortune Feimster voiced the lesbian characters Deb and Louisa Desk.
 * Steven Universe:
 * Ruby and Sapphire are feminine sexless aliens romantically involved with each other. Sapphire's voice actress, Erica Luttrell, is WLW.
 * Non-binary Drag Queen Jinkx Monsoon voices Emerald, who is sexless and female-presenting.
 * Steven Universe: Future: Shep is a transgender, non-binary character using "they/them" pronouns, similarly to their voice actor Indya Moore.
 * The Owl House:
 * Raine Whispers is non-binary and uses "they/them" pronouns, just like their voice actor Avi Roque.
 * Amity Blight is lesbian and her voice actress Mae Whitman came out as pansexual in August 2021.